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. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: Hepatology. 2023 Apr 1;78(2):503–517. doi: 10.1097/HEP.0000000000000373

Figure 4. Loss of hepatic Tsc1 causes AH-like cell population reprogramming in EtOH-fed mouse livers.

Figure 4.

Male Tsc1 WT and L-Tsc1 KO mice were fed with Gao-binge alcohol. (A) Heat map of gene expression signatures for hepatocyte, cholangiocyte, HSC and Kupffer cells from qRT-PCR analysis. RNA was extracted from mouse livers followed by qRT-PCR. Results were normalized to 18s and expressed as fold change compared with Tsc1 WT Ctrl group (n=4). (B) Total liver lysates were subjected to western blot analysis. Data are densitometry analysis and presented as means ± SE (n= 3-4). (C) qRT-PCR analysis of YAP1 target gene expression. Data are presented as means ± SE (n=5-8). *p<0.05; One-Way ANOVA analysis with Bonferroni post hoc test. (D) Representative images of IHC staining for SOX9, YAP1 and HNF4α in mouse livers of indicated genotypes and feeding. Arrow denote SOX9 and YAP1 positive ductular cells, and arrowhead denotes HNF4α negative ductular cells.