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. Author manuscript; available in PMC: 2024 Aug 1.
Published in final edited form as: Hepatology. 2023 Apr 1;78(2):503–517. doi: 10.1097/HEP.0000000000000373

Figure 7. Inhibition of mTORC1 meliorates liver injury, hepatomegaly, DR, fibrosis, and inflammatory cell infiltration in EtOH-fed L-Tsc1 KO mouse livers.

Figure 7.

Male L-Tsc1 KO mice were fed with Gao-binge alcohol. Five doses Torin1 or vehicle control were given to mice during the 10 days alcohol feeding (day 1, 3, 5, 7 and 9, i.p. 2 mg/kg), and one dose was given right before the gavage on the final day. (A) Serum ALT activity, (B) liver weight versus body weight ratio, and (C) hepatic TG were analysis. Data are means ± SE (n=4-6). *p<0.05; Student’s t-test. (D) Total liver lysates were subjected to western blot and densitometry analysis. Data are presented as means ± SE (n= 3). (E-I) Representative photographs and quantification data of H&E, CK19, F4/80, MPO and Sirius Red staining are shown. More than 20 different fields (20x) from each mouse were quantified in a blinded fashion. Data are presented as means ± SE (from n=4-6 mice). *p<0.05; Student’s t-test. V: vehicle; Tor: Torin1.