Figure 3.

No excitatory or inhibitory neuron loss occurs at time of perisomatic synapse loss

(a) In situ hybridization (ISH) for Vglut1 and Gad1 in layer V of mock- and Toxoplasma gondii (ME49)-infected neocortex reveals no significant decrease in the number of excitatory or inhibitory cells following infection.

(b) Quantification of Vglut1⁺ cell count in layer V of mock- and ME49-infected cortex. Each data point represents one biological replicate and bars depict mean ± standard error of the mean (SEM). No significant difference (ns) indicates P > 0.05 by Student’s one-tailed t-test (n=3 mice per condition). P-value = 0.1030; t = 1.508; df = 4. Normality assessed by F-test (F value = 1.518, P value = 0.7944, not significant).

(c) Quantification of Gad1⁺ cell count in layer V of mock- and ME49-infected cortex. Each data point represents one biological replicate and bars depict mean ± SEM. No significant difference (ns) indicates P > 0.05 by Student’s one-tailed t-test (n=3 mice per condition). P-value = 0.2249; t = 0.8367; df = 4. Normality assessed by F-test (F value = 6.778, P value = 0.2571, not significant).

Scale bar in A: 20 μm