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. Author manuscript; available in PMC: 2025 Oct 1.
Published in final edited form as: J Neurochem. 2023 Feb 17;168(10):3365–3385. doi: 10.1111/jnc.15770

Figure 5.

Figure 5.

Complement mRNA is differentially upregulated by immune and neuronal cells in the infected brain

(a) In situ hybridization (ISH) for C1qa and IHC for IBA1 in layer V of neocortex of mock- and Toxoplasma gondii (ME49)-infected mice.

(b) ISH for C3 and IHC for IBA1 in layer V of neocortex of mock- and ME49-infected mice.

(c) ISH for Syt1 and C1qa showed no neuronal expression of C1qa mRNA in both mock- and ME49-infected layer V.

(d) ISH for Syt1 and C3 in mock- and ME49-infected mice. Neuronal expression of C3 mRNA was detected in some neurons in ME49-infection.

(e) ISH for Npnt and C3 in mock- and ME49-infected mice. C3 mRNA was detected in some Npnt-expressing pyramidal neurons in layer V of neocortex.

(f) ISH for Gad1 and C3 show no expression of C3 mRNA by inhibitory neurons in ME49-infection.

Scale bars in A - F: 10 μm