FIG. 7.

Autoradiogram of in vitro strand exchange assay using artificial dif Holliday junction substrate labeled with ³²P (∗). The exchange of top strands generated a 76-bp linear duplex product, while strand cleavages generated covalently bound recombinase-DNA molecules of either the dif-HJ substrate (HJCC-MBP) or a linear duplex product (LDCC-MBP). Note the high levels of product and covalent complexes in the reaction mixture containing CodV and RipX (lane 9). The inclusion of a specific protein in a reaction mixture is denoted by a plus in the grid below the gel.