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. Author manuscript; available in PMC: 2023 Jul 23.
Published in final edited form as: Nat Methods. 2023 Mar 9;20(5):677–681. doi: 10.1038/s41592-023-01816-z

Extended Data Fig.9.

Extended Data Fig.9.

Effect of light illumination on the viability and growth rate of the spheroids. a, Fluorescent images of the spheroids in control and illuminated groups. Live cells fluoresce in green (Calcein-AM) and dead cells fluoresce in red (EthD-1). b, Growth rate of the spheroids is not affected by laser illumination. The upper panel is the representative time-lapse image of a spheroid that is illuminated on Day 2. The points in the lower panel represent the area of spheroids over time. Black curves are best fit for exponential growth. Control group (n=10), Illuminated group (n=7).