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. 2023 Jul 19;18:3951–3972. doi: 10.2147/IJN.S410996

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© 2023 He et al.

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Cytotoxicity evaluation of the TTO-NL@PCS sustained-release nanosystem. (A) Live and dead staining of keratinocytes (KCs) cultivated with electrospun nanofibers on days 1 and 3; (B and C) viability and cell number analyses (green, viable cells; red, dead cells). (D) Phalloidin and DAPI labeling of cell proliferation on days 1 and 3, and (E) cell area quantification of KC proliferation on various electrospun surfaces. *P < 0.05, scale bars =100 m, n = 3 (random field of vision). (F) HaCaT and HUVEC cells co-cultured with equal volumes of TTO-NL@PCS, TTO@PCS, and PCS after 24 h (n = 3).