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. 2023 Jul 19;18:3951–3972. doi: 10.2147/IJN.S410996

Figure 4.

Figure 4

Inhibition zone assays and analysis of MRSA biofilm-dispersion and bactericidal abilities of electrospun nanofibers. (A) Inhibition zone assay results of electrospun nanofibers placed on bacteria (ATCC 43300) spread on agar plates (scale bar 1 cm), with (D) quantification of inhibition zone assays. (B) Live and dead staining results of bacteria (ATCC 43300) cultured with electrospun nanofibers at preset timepoints (scale bar 50 µm), with (E) quantification results of the staining results. *P < 0.05, TTO-NL@PCS vs TTO@PCS (6 h and 12 h). (C) Residual MRSA biofilms following treatment with electrospun nanofibers at day 1 and day 3 were stained with crystal violet and processed by ImageJ software (scale bar, 100 µm). (F) Relative MRSA biofilm biomass treated with different materials. MRSA biofilm biomass was determined by measuring the absorbance at 590 nm. (G) LSCM 3-D schematic diagram of LIVE/DEAD-stained MRSA biofilm grown on cell slides (green, viable cells; red, dead cells), and (H) quantification of the relative immunofluorescence intensity per field on day 1 following various electrospun nanofiber treatments. n = 3 (random field of vision), *P < 0.05.