Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 19;16:3019–3032. doi: 10.2147/JIR.S413358

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Song et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

The effect of anti-CA1 and anti-CA2 siRNA on VSMC calcification. VSMCs were transfected with anti-CA1 and anti-CA2 siRNA and cultured in M1 macrophage supernatant and calcification-inducing medium (1:1) (ANOVA, *Stands for p<0.05, **Stands for p<0.01, ***Stands for p <0.001, and ****Stands for p <0.0001). (a) Real-time PCR indicated the successful inhibition of target gene expression in VSMCs by anti-CA1 and anti-CA2 siRNAs. (b) AR-S staining showed few calcified nodules in VSMCs that were transfected with anti-CA1, and M1 culture supernatants partially restored calcification levels (the location of calcification nodules was marked with black arrows). Quantification of calcification with a cetylpyridine chloride assay verified this result. (c) AR-S staining showed few calcified nodules in VSMCs that were transfected with anti-CA2 siRNA, and M1 culture supernatants partially restored calcification levels (the location of calcification nodules was marked with black arrows). Quantification of calcification with a cetylpyridine chloride assay verified this result. CA1 and CA2 expression plays an essential role in the calcification of VSMCs.