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. 2023 Jul 19;16:3019–3032. doi: 10.2147/JIR.S413358

Figure 5.

Figure 5

The effect of anti-CA1 and anti-CA2 siRNA on CA1 and CA2 expression in VSMCs and proinflammatory cytokine production (ANOVA, *Stands for p<0.05, **Stands for p<0.01, ***Stands for p <0.001, and ****Stands for p <0.0001). (a) CA1 expression in VSMCs was examined by real-time PCR. (b) CA1 expression in VSMCs was examined by Western blotting. (c) CA2 expression in VSMCs was examined by real-time PCR. (d) CA2 expression in VSMCs was examined by Western blotting. Anti-CA1 or anti-CA2 siRNA treatment decreased CA1 and CA2 expression in VSMCs, respectively, and M1 supernatant alleviated the suppressive effects of anti-CA1 and anti-CA2 siRNA on the target genes. (e) The levels of IL-6, IL-1β and TNF-α in the culture medium of VSMCs after transfection with anti-CA1. (f) The levels of IL-6, IL-1β and TNF-α in the culture medium of VSMCs after transfection with anti-CA2. Anti-CA1 and anti-CA2 siRNA transfection reduced IL-6, IL-1β and TNF-α levels in the culture, and M1 supernatant alleviated the suppressive effects of anti-CA1 and anti-CA2 siRNA on proinflammatory cytokine production.