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OMs of E. coli expressing different HemR histidine mutants from a pWKS130 plasmid. Lanes: 1, HemRSTOP (pNEO1391 [see Table 1]); 2, E. coli DH5α; 3, HemRH352K; 4, HemRH461L; 5, HemRH192T; 6, HemRH128K; 7, wild-type HemR; M, molecular mass markers (30, 46, 69, and 94 kDa).
