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Ability of E. coli EB53 (hemA aroB) cells expressing HemR mutant receptors to use BSA-heme complexes (10 μM BSA and 1 μM heme) (A) and HSA-heme complexes (50 μM heme) as sources of heme. HemRW.T, wild-type HemR; HemRSTOP, pNEO1391. Two to three independent growth measurements were conducted, producing essentially identical results.
