TABLE 1.
Bacterial strains and plasmids used in the study
| Bacterial strain or plasmid | Relevant genotypea | Source or reference |
|---|---|---|
| Escherichia coli | ||
| DH5α | Stratagene | |
| IR1532 | DH5α but heme biosynthesis mutant | 47 |
| IR1583 | As IR1532 but tonB exbB exbDNM | 47 |
| EB53 | E. coli hemA aroB Rifr | 43 |
| IR754 | EB53 but tonB::Kanr | 45 |
| Y. enterocolitica WA-C | ||
| H1883 | Plasmidless strain, hemPRSTVU+ | 43 |
| H5030 | hemR::phoA Ampr | 43 |
| PLASMIDS | ||
| pT76.91 | pT7-5, hemPR | 43 |
| pHEM101 | pBluescript hemPR | 43 |
| pT76.53 | hemPRST′ | 43 |
| pWKS130 | Low-copy-number vector, Neor | 49 |
| pWKS30 | Low-copy-number vector, Ampr | 49 |
| pAMP1173 | pWKS30 carrying hmbRWT | This study |
| pNEO76.91 | pWKS130, hemRWT | Laboratory collection |
| pNEO1187 | pWKS130, hemRH128K | This study |
| pNEO1188 | pWKS130, hemRH461K | This study |
| pNEO1194 | pWKS130, hemRH352K | This study |
| pWSH1332 | pWKS130, hemRH192T | This study |
| pNEO1391 | pWKS130, hemRSTOP | This study |
| pICE1403 | pWKS130, hemRH192T,H352K | This study |
| pNEO1505 | pWKS130, hemRH352A | This study |
| pNEO1507 | pWKS130, hemRH461A | This study |
| pCSB1707 | pWKS130, hemRH128A,H461A | This study |
a
hemRWT, wild-type hemR.