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Overview of the ΔRT shift based on the recombinant citrullinated peptides. (a) Base-peak chromatograms of nonmodified, monomodified, and double-modified citrullinated peptide with (b) different times and gradients of ACN. Each peptide was run three times under the same condition (c) Table of ΔRT calculations between each peptide with final gradients of ACN from 3 to 35% at different time increments.
