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. 2023 Jun 9;64:102782. doi: 10.1016/j.redox.2023.102782

Fig. 4.

Fig. 4

CircRNA Galntl6 bound to miR-335 and decreased its expression. (A) Predicted binding sites between circRNA Galntl6 and miR-335. (B) RNA pulldown assay showed that biotin-labeled miR-335 interacted with circRNA Galntl6. (C) Dual-luciferase reporter assay was used to confirm the targeted relationship between circRNA Galntl6 and miR-335. (D and E) RT-qPCR was used to measure the effect of circRNA Galntl6 overexpression or knockdown on miR‐335 expression in B104 cells. (F) Relative expression of miR-335 was detected by RT-qPCR in RVLM administrated with pLV-circRNA Galntl6 plasmid. Data were expressed as mean ± SEM. Statistical significance was determined by unpaired two-tailed Student's t-test (B–E) and one-way ANOVA, followed by post-hoc Bonferroni test (F). n = 6 of independent cell culture preparations (B–E). n = 6 rats per group (F). ##p < 0.01, ###p < 0.001, and ns means nonsignificant versus agomir NC group. ΔΔp < 0.01 versus pLV-NC group. &&p < 0.01 versus si-NC group. **p < 0.01, and ns means nonsignificant versus SIH group.