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. 2023 Jun 9;64:102782. doi: 10.1016/j.redox.2023.102782

Fig. 6.

Fig. 6

Lig3 was a downstream mRNA target of miR-335. (A) Schematic graph of the potential binding sites for miR-335 within Lig3 3′ UTR. (B) The luciferase activities of Lig3 luciferase reporters (WT or MUT) were tested in B104 cells co-expressing miR-335 agomir or agomir NC. (C and D) RT-qPCR and Western blot analyses showed that the expression of Lig3 decreased or increased in B104 cells when miR-335 was overexpressed or downregulated, respectively. (E) Following miR-335 knockdown in RVLM of SIH rats, Lig3 expression was measured using RT-qPCR and Western blot assays. Data were expressed as mean ± SEM. Statistical significance was determined by unpaired two-tailed Student's t-test (B–D) and one-way ANOVA, followed by post-hoc Bonferroni test (E). n = 3–6 of independent cell culture preparations (B–D). n = 3–6 rats per group (E). ##p < 0.01, ###p < 0.001, and ns means nonsignificant versus agomir NC group. Δp < 0.05, and ΔΔp < 0.01 versus antagomir NC group. **p < 0.01, and ***p < 0.001 versus SIH group.