Fig. 2.

Semaglutide reduces the intake of food in alcohol drinking male and female rats. In male rats, semaglutide at both doses, (A) 0.026 mg/kg (n = 12) and (B) 0.052 mg/kg (n = 12) reduce the overall food intake compared to vehicle (n = 12 in each test); a reduction evident at each treatment session. (C) Compared to vehicle (n = 12), repeated semaglutide treatment (0.026 mg/kg) lowers the overall intake of food in male rats. This decline is found at each treatment session. Similarly, compared with vehicle (n = 12 in each test) both semaglutide doses, (D) 0.026 mg/kg (n = 12) and (E) 0.052 mg/kg (n = 12), decrease food intake at each treatment drinking session in female rats. (F) Additionally, in comparison to vehicle (n = 12), repeated semaglutide treatment (0.026 mg/kg, n = 12) decreases the food intake in females, a reduction evident at alcohol drinking session 1, 2, 3 and 5. Overall interaction effect (A–F) from two-way ANOVA with repeated measures is stated in the figure. Experiments were not replicated Data are presented as mean ± SEM, significant data are illustrated by ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. Syringe indicates time of injection.