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. 2023 Jun 19;93:104650. doi: 10.1016/j.ebiom.2023.104650

Fig. 1.

Fig. 1

UCKL1 is upregulated in CRC and associated with poor prognosis. (a) Genomic alterations of pyrimidine metabolism genes in CRC database (TCGA, Firehose Legacy) analysed by cBioPortal for Cancer Genomics. (b) Schematic map of pyrimidine synthesis pathway. (c) Genomic alterations of UCKL1, UCK1 and UCK2 in CRC database analysed by cBioPortal for Cancer Genomics. (d) Waterfall plot of the relative UCKL1 mRNA expression levels from 30 paired samples of CRC and adjacent normal tissue. N, adjacent normal tissue; T, tumour tissue. (e) UCKL1 protein level was detected from paired samples of CRC and adjacent normal tissue. N, adjacent normal tissue; T, tumour tissue. (f) Representative images of UCKL1 IHC staining in human CRC and adjacent normal tissue in cohort 1 and cohort 2. Scale bars: 25 μm. Image J was used to quantify the intensity of the staining, and z-score was calculated to evaluate the expression of UCKL1. Mann–Whitney U test and Unpaired two-tailed Student’s t-test were used to compare the results. (g) Kaplan–Meier survival curves based on UCKL1 expression in the CRC tissue from TCGA database.