Fig. 2.

Plasma NfL levels (a) and predicted years to 60% probability of clinical motor diagnosis (yCMD) (b) differed between HD-ISS stages and healthy controls (HC). Comparisons conducted using Kruskal–Wallis test with Dunn’s post-hoc comparisons. Error bars represent median and interquartile range; ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05. (c) Depicts the relationship between yCMD and plasma NfL levels, including our proposed cut-point of 45.0 pg/ml (dotted lined in x-axis) for identifying participants with less than ten yCMD (dotted line in y-axis), and the HD-ISS. Sample sizes: Stage 0 (S0) = 50, Stage 1 (S1) = 64, Stage 2 (S2) = 63, Stage 3 (S3) = 64, Healthy Controls (HC) = 50. All measurements conducted with two biological replicates.