Fig. 3.

Plasma NfL levels differed significantly by HD-ISS categorisation method (original HD-ISS thresholds [HD-ISS] versus PIN-substituted thresholds [PIN]) for Stage 3 (S3), but not Stage 2 (S2) or Stage 0 and Stage 1 combined (≤S1). Error bars represent median and interquartile range. The dotted line indicates our proposed plasma NfL cut-point (45.0 pg/ml) for <10 predicted years to clinical motor diagnosis. Sample sizes: ≤Stage 1 (≤S1) (PIN) = 114, ≤S1 (HD-ISS) = 109; Stage 2 (S2) (PIN) = 63; S2 (HD-ISS) = 31; Stage 3 (S3) (PIN) = 67, S3 (HD-ISS) = 100. All measurements conducted with two biological replicates.