Fig. 1.
Long-term culture of HCASMC under physiological normoxia (5 kPa O2) upregulates calponin-1 expression
A and C, Representative immunofluorescence images of SM22-α or calponin-1 and DAPI stained nuclei in HCASMC pre-adapted for 5d to 18 or 5 kPa O2. B and D, Representative immunoblots and densitometric analysis of SM22-α and calponin-1 expression relative to β-actin. Data denote mean ± S.E.M., n = 3–6 independent cell cultures (color-coded), unpaired Student's t-test, **P < 0.01. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)