Fig. 2.

Proliferation of HCASMC during long-term culture under 18 or 5 kPa O₂

HCASMC were pre-adapted for 5d to either 18 or 5 kPa O₂. A-B, Cells seeded at 3500 cells/well into 96-well plates and cell number counted and total protein content measured over 1–5 days in culture. C, Cells seeded at 7000 cells/well in E-Plates® and doubling time monitored continuously over 6 days using an iCELLigence platform. Data denote mean ± S.E.M., n = 3–4 independent cultures (color-coded), two-way ANOVA followed by a Bonferroni Post Hoc test analysis, *P < 0.05, **P < 0.01, ***P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)