Fig. 4.

Zn supplementation induces NRF2 nuclear accumulation in HCASMC under 18 or 5 kPa O₂

A, Representative NRF2 positive immunofluorescence and DAPI stained nuclei in HCASMC pre-adapted to18 or 5 kPa O₂ and then treated for 16 h with Veh (0.01% DMSO), TPEN (1.25 μM) or ZnCl₂ (10 μM) + pyrithione (Py, 0.5 μM), respectively. B, Quantification of NRF2 nuclear:cytoplasmic ratio in HCASMC treated with Veh, TPEN or ZnCl₂+Py. Data denote mean ± S.E.M., n = 3–4 independent cultures (color-coded) with 20–30 cells analyzed in each culture, two-way ANOVA followed by a Bonferroni Post Hoc test analysis, **P < 0.01, ***P < 0.001. Scale bar = 20 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)