Fig. 5.
Effect of Zn supplementation on HO-1 and NQO1 mRNA and protein expression in HCASMC under 18 or 5 kPa O2
HCASMC were pre-adapted for 5d to 18 or 5 kPa O2. A and B, mRNA expression of HO-1 and NQO1 in HCASMC treated for 6 h with Veh (0.01% DMSO), TPEN (1.25 μM) or ZnCl2 (10 μM) + pyrithione (Py, 0.5 μM), respectively. Values normalized to three housekeeping genes (TBP, RPLPO and SDHA). C and D, Representative immunoblots and densitometric analysis of HO-1 and NQO1 expression relative to β-actin following treatment for 16 h with Veh (0.01% DMSO), TPEN (1.25 μM) or ZnCl2 (10 μM) + pyrithione (Py, 0.5 μM). Data denote mean ± S.E.M., n = 4–6 independent cultures (color-coded), two-way ANOVA followed by Bonferroni's multiple comparisons test, *P < 0.05, ***P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)