Fig. 6.
Reoxygenation induced reactive oxygen species generation in HCASMC under 18 or 5 kPa O2
A and C, Representative L-012 luminescence traces in HCASMC pre-adapted for 5d to 18 or 5 kPa O2. Cells were treated with Veh (0.01% DMSO), PEG-superoxide dismutase (pSOD, 20U/ml) or PEG-catalase (pCAT, 200U/ml). Cells were then incubated with L-012 and transferred to an O2-regulated plate reader gassed with 18 or 5 kPa O2, respectively. L-012 luminescence was detected in the plate reader, with O2 reduced to 1 kPa and reoxygenation under 18 or 5 kPa O2, respectively. The red line indicates the pericellular O2 levels within the plate reader. B and D, Area under curve summary of reoxygenation-induced L-012 luminescence changes. L-012 signal in panel B (18 kPa O2) denotes area under the curve x106 and in panel D (5 kPa O2) area under the curve x104. Data denote mean ± S.E.M., n = 6 independent cultures, one-way ANOVA followed by Bonferroni's multiple comparisons test, *P < 0.05, ***P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)