Fig. 7.

Zn supplementation attenuates reoxygenation-induced reactive oxygen species generation in HCASMC under 18 kPa O₂

A and C, Representative L-012 luminescence traces in HCASMC pre-adapted for 5d to 18 or 5 kPa O₂. Cells were treated for 16 h with Veh (0.01% DMSO), TPEN (1.25 μM) or ZnCl₂ (10 μM) + pyrithione (Py, 0.5 μM). Cells were then incubated with L-012 and transferred to an O₂-regulated plate reader. L-012 luminescence was detected in the plate reader gassed with 18 or 5 kPa O₂, with O₂ then reduced to 1 kPa and reoxygenation under either 18 or 5 kPa O₂, respectively. The red line indicates the pericellular O₂ levels within the plate reader. B and D, Area under curve summary of reoxygenation-induced L-012 luminescence changes. L-012 signal in panel B (18 kPa O₂) denotes area under the curve x10⁶ and in panel D (5 kPa O₂) area under the curve x10⁴. Data denote mean ± S.E.M., n = 4 independent cultures, one-way ANOVA followed by Bonferroni's multiple comparisons test, *P < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)