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. 2023 Jan 21;4(4):100553. doi: 10.1016/j.xplc.2023.100553

Figure 5.

Figure 5

Expression patterns of ZmLAC3 and ZmLAC5 in an area 1–2 cm from the root apex.

(A) Accumulation of ZmLAC3 and ZmLAC5 transcripts in the root tip of maize grown hydroponically under ND as determined by in situ hybridization analysis. Anti-sense and sense probes on the images indicate the positive and negative signals, respectively. Scale bars represent 100 μm.

(B) Responses of ZmLAC3 and ZmLAC5 to N treatments as determined by RNA-seq analysis and qRT-PCR. For RNA-seq analysis, the fragments per kilobase of transcript per million mapped reads value was used to determine normalized gene expression. For qRT-PCR, the expression levels were normalized to that of ZmUBQ12, and the levels of ZmLAC3 and ZmLAC5 transcripts under NL were set to 1.0. Error bars represent the SE of three independent experiments. ∗∗P < 0.01 (Student’s t-test) indicates a significant difference between NL and ND. NL and ND indicate N luxury and N deficiency, respectively.