Fig. 3.

Dioscin mediates demethylation in hypertrophic hearts. C57BL/6 mice were subjected to sham or TAC operations. Mice were treated with dioscin or vehicle for 4 weeks from day 1 after operation. (A) DNMT activities were determined in heart tissues from each group (n = 6 per group; *P < 0.05 vs. Sham, #P < 0.05 vs. TAC). (B) Representative images of fluorescence staining for mC staining (green) were presented, and TnT was as cardiomyocytic marker (red). (C-G) AngII-incubated NMCMs were transfected with siRNA against Dnmt1 and then treated with dioscin. (C) DNMT activities were determined in different treated cells. (D) The mRNA levels of Anp, Bnp and β-Mhc were determined by qPCR assay in different groups. WGA staining (E) and active MMPs staining (F) were presented (n = 4 per group). (G) Real-time PCR analysis was performed to detect the mRNA levels of Mmp2, Mmp9, Col1, and Col3 genes in NMCMs, n = 6 per group. *P < 0.05 vs. Control, #P < 0.05 vs. AngII + Veh. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)