FIG. 1.

Images of M. capricolum cells observed by phase-combined fluorescence microscopy. Cells were fixed, stained with DAPI, and photographed. (A) Culture was grown to an OD₆₀₀ of 0.05 in the standard medium. (B) Culture was grown to an OD₆₀₀ of 0.4 without nucleoside supplementation. (C and D) Cells grown to an OD₆₀₀ of 0.05 in the standard medium were transferred to a medium without supplementation for lipid synthesis and incubated for 1 and 2 h, respectively. (E) Cells grown to an OD₆₀₀ of 0.05 in the standard medium were incubated with chloramphenicol at 37°C for 1.5 h. Bar, 2 μm. (F) Schematic illustration of M. capricolum cells. a, cell length; b and c, nucleoid positions. Internucleoid distance was calculated by subtraction of b from c.