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. 2023 Mar 2;4(4):100570. doi: 10.1016/j.xplc.2023.100570

Figure 1.

Figure 1

HOS15-impaired mutants are insensitive to low ambient temperature–mediated repression of flowering.

(A) Effect of low ambient temperature on flowering of wild-type Col-0 (WT), hos15-2, hos15-i, and proHOS15:HOS15-HA hos15-2 (HOS15HA). Plants were grown under LDs (16L/8D) at 23°C or 16°C. The photographs were taken when hos15-2 and hos15-i mutants bolted. Scale bars correspond to 1 cm.

(B) The numbers of rosette and cauline leaves were counted at bolting to measure flowering time. Data are presented as means ± SD of three independent replicates, each including at least 15 plants. Different letters denote statistically significant differences (ANOVA, Bonferroni post-hoc test, P < 0.05).

(C) Transcript levels of GI, CO, SOC1, and FT at low ambient temperatures. Twelve-day-old plants at 23°C or 16°C in LDs were harvested every 4 h, and the transcript levels of GI, CO, SOC1, and FT were measured by quantitative real-time PCR. Three independent replicates were averaged, and error bars represent the SD of the mean. Asterisks indicate statistically significant differences between 23°C and 16°C in WT and hos15-2 (Student’s t-test; ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05).