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. 2023 Mar 2;4(4):100570. doi: 10.1016/j.xplc.2023.100570

Figure 4.

Figure 4

hos15-2 has a defect in GI degradation induced by low ambient temperature but not by darkness.

(A) GI protein levels in gi-2 35S:GI-HA, hos15-2 35S:GI-HA, and cop1-4 35S:GI-HA in response to low ambient temperature, darkness, and a combination of conditions after cycloheximide treatment. Plants were grown for 10 days under 12L/12D conditions at 23°C and then transferred to 16°C, darkness, and a combination of conditions at ZT12 with cycloheximide treatment. A schematic diagram shows the experimental process.

(B) Quantification of GI amount under each condition relative to the GI amount under continuous light at 23°C. The levels of GI-HA protein under each condition were normalized to that of HSP90 and compared with the levels under the 23°C light condition. Data are presented as means ± SD of three independent replicates. Different letters denote statistically significant differences among gi-2 35S:GI-HA, hos15-2 35S:GI-HA, and cop1-4 35S:GI-HA under the same experimental conditions at the same time point (two-way ANOVA, Bonferroni post-hoc test; P < 0.05).