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. 2023 Mar 2;4(4):100570. doi: 10.1016/j.xplc.2023.100570

Figure 6.

Figure 6

HOS15 is genetically dependent on COP1 in low ambient temperature–mediated flowering but independent of COP1 in low ambient temperature-induced GI turnover.

(A) Flowering phenotypes of WT, hos15-2, cop1-4, and hos15-2 cop1-4 mutants grown under LDs (16L/8D) at 23°C or 16°C. The photographs were taken when hos15-2 bolted. Scale bars correspond to 1 cm.

(B) The numbers of rosette and cauline leaves were counted at bolting to measure flowering time. Data are presented as means ± SD of three independent replicates, each including at least 15 plants. Different letters denote statistically significant differences (ANOVA, Bonferroni post-hoc test; P < 0.05).

(C and D) GI protein levels in hos15-2, cop1-4, and hos15-2 cop1-4 plants at 23°C (C) or 16°C (D). Plants were grown for 10 days under 12L/12D conditions at 23°C and then transferred to 16°C for an additional 2 days.

(E) Quantification of GI protein levels in hos15-2 35S:GI-HA, cop1-4 35S:GI-HA, and hos15-2 cop1-4 35S:GI-HA. The levels of GI-HA protein were normalized to HSP90 and statistically analyzed. Data are presented as means ± SD of three independent replicates. Different letters denote statistically significant differences (ANOVA, Bonferroni post-hoc test; P < 0.05).