Figure 3.

MoMsn2 targets fatty acid β-oxidation-related genes.

(A) EMSAs for binding of MoMsn2 protein with the putative promoter sequences of MGG_07019, MoPOX1, and MoDCI1. The 1500-bp DNA fragment of the putative promoter sequence of each gene was incubated in the absence or presence of purified MoMsn2 protein. pET-32a-His empty protein or proteinase K was added after incubation of MoMsn2 protein with the DNA fragment as a control. DNA–protein complexes were separated by 1.5% agarose gel electrophoresis and photographed. His-T, tag protein (hexahistidine and Trx) expressed by the empty vector pET-32a.

(B) EMSAs with increased amounts of MoMsn2 protein.

(C) Luciferase activity assay. The tested constructs were co-expressed or separately expressed in N. benthamiana. Luciferase activity was detected after 48 hpi. P1, negative control; P3, positive control.