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. 2023 Feb 11;4(4):100561. doi: 10.1016/j.xplc.2023.100561

Figure 5.

Figure 5

Endocytosis mediated by MoLrp1 is important for MoMsn2 accumulation from the cytosol to the nucleus under Ole treatment.

(A and B) Hyphae of wild-type Guy11 and the ΔMolrp1 mutant co-expressing MoMsn2-GFP and H1-RFP proteins were observed under a confocal microscope after treatment with different fatty acids or NaCl for 10 or 30 min. NaCl treatment was used as a positive control. Insets highlight areas analyzed by line scan. The green line represents MoMsn2-GFP; the red line represents H1-RFP. Scale bar, 10 μm.

(C) Hyphae of Guy11, ΔMolrp1, and the complemented transformant MoLRP1-C with or without chlorpromazine (Chl) treatment were stained with FM4-64 and examined under a confocal microscope. Insets highlight areas analyzed by line scan. Scale bar, 10 μm.

(D) Hyphae of Guy11 and ΔMolrp1 co-expressing MoMsn2-GFP and H1-RFP proteins were co-treated with Chl and Ole for 10 min and observed under a confocal microscope. Insets highlight areas analyzed by line scan. The green line represents MoMsn2-GFP; the red line represents H1-RFP. Scale bar, 10 μm.