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. 2023 Mar 20;4(4):100592. doi: 10.1016/j.xplc.2023.100592

Figure 2.

Figure 2

Identification of SHCTs that convert salidroside into osmanthuside A.

(A) Reactions catalyzed by SHCT and OART. (1) salidroside; (2) osmanthuside A; (3) syringalide A; (4) osmanthuside B; (5) syringalide A 3′-α-L-rhamnopyranoside.

(B) Percentage protein identities of LrAT1 homologs.

(C) Expression profiles of LrAT1 and OART homologs across different samples of P. barbatus, R. glutinosa, and S. indicum. Pearson correlation coefficients of pairs of LrAT1 and OART homologs are shown.

(D) HPLC analysis of reaction mixtures of LrAT1 homologs and RgUGT79G15 using p-coumaroyl-CoA as an acyl donor.

(E) HPLC analysis of reaction mixtures of SiAT1 and RgUGT79G15 using caffeoyl-CoA as an acyl donor.

(F) Michaelis-Menten kinetics of SiAT1 with p-coumaroyl-CoA and caffeoyl-CoA. Means of triplicates and standard deviations are shown.