Figure 2.

The production pathways of various GHPs catalyzed by myrosinases and governed by different specifier proteins.

The unstable intermediate aglycones produced by myrosinases are enclosed in square brackets.(A) Catalyzed by TGG-encoding myrosinases, an intermediate aglycone is produced, which can spontaneously convert into an isothiocyanate or further cyclize to form an oxazolidine-2-thione at typical cell pH.(B) The pathways of simple nitrile production in the presence of ESPs or NSPs.(C) The production of an epithionitrile from an alkenyl aglucone in the presence of ESPs.

(D) The production of a thiocyanate from an allyl, a 4-methylthiobutyl, or a benzyl aglycone in the presence of TFPs.

Information sources: Lüthy and Benn (1977); Bones and Rossiter (1996); Burow et al. (2006, 2008); Kuchernig et al. (2011); Agerbirk and Olsen (2012); Gumz et al. (2015); Wittstock et al. (2016a, 2016b).