Table 3.
Phenotypic alterations following mutagenesis of genes involved in the GSL core structure assembly step.
| Locus | Strategy | Species | Plant growth and agronomic traitsa |
GSL contenta |
Tissue/organ | Benefit | Reference | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Height | Fertility | Yield | Survival | Met-derived | Indole | ||||||
| CYP79F1 | KO | A. thaliana | – | – | – | –/+/– | +/N/+ | leaf/root/seed | Reintanz et al. (2001) | ||
| OE | B. rapa | – | + | N | leaf | Zang et al. (2008) | |||||
| CYP79F2 | KO | A. thaliana | N | N | N | N | +/N/N | leaf/root/seed | Chen et al. (2003) | ||
| KO | A. thaliana | N | N | N | N | –/+/–/– | N/+/+/+ | leaf/stem/root/flower | Tantikanjana et al. (2004) | ||
| OE | A. thaliana | – | N | leaf/root/seed | Chen et al. (2003) | ||||||
| CYP79F1CYP79F2 | KO | A. thaliana | – | – | – | – | +/+/–/+ | leaf/stem/root/flower | Tantikanjana et al. (2004) | ||
| CYP79B2CYP79B2 | OE | A. thaliana | – | – | N | + | leaf | resistance to Pseudomonas syringae pv. maculicola |
Hull et al. (2000); Mikkelsen et al. (2000) |
||
| KO | A. thaliana | N | N | N | N | Zhao et al. (2002) | |||||
| CYP79B3CYP79B3 | KO | A. thaliana | N | N | N | N | Zhao et al. (2002) | ||||
| OE | B. rapa | N | root | Zang et al. (2009) | |||||||
| CYP79B2CYP79B3 | KO | A. thaliana | – | – | Zhao et al. (2002) | ||||||
| CYP83A1 | KO | A. thaliana | N | N | N | N | +/N | leaf/seed | resistance to Erysiphe cruciferarum, Glovinomyces orontii, and G. cichoracearum |
Hemm et al. (2003); Weis et al. (2013), 2014; Liu et al. (2016a) |
|
| OE | A. thaliana | N | N | N | N | N | N | seedling/leaf | Naur et al. (2003) | ||
| OE | B. napus | + | N | leaf/seed | Zhang et al. (2015b) | ||||||
| CYP83B1 | KO | A. thaliana | – | – | – | seedling |
Bak et al. (2001); Bak and Feyereisen (2001) |
||||
| OE | A. thaliana | – | – | + | seedling | Bak and Feyereisen (2001) | |||||
| OE | A. thaliana | – | + | N | leaf | Naur et al. (2003) | |||||
| CYP79B2CYP79B3CYP83B1 | OE | B. rapa | N | root | Zang et al. (2009) | ||||||
| GSTF11 | KO | A. thaliana | N | N | N | N | – | N | leaf/seed | Zhang et al. (2022) | |
| GSTU20 | KO | A. thaliana | N | N | N | N | – | N | leaf/seed | Zhang et al. (2022) | |
| GSTF11GSTU20 | KO | A. thaliana | – | N | leaf/seed | Zhang et al. (2022) | |||||
| GGP1GGP3 | KD | A. thaliana | – | + | leaf | Geu-Flores et al. (2011) | |||||
| SUR1 | KO | A. thaliana | – | – | – | – | – | shoot | Mikkelsen et al. (2004); Grubb et al. (2014) | ||
| OE | A. thaliana | N | N | N | N | N | N | Mikkelsen et al. (2004) | |||
| UGT74B1 | KO | A. thaliana | – | – | – | – | – | leaf/shoot |
Grubb et al. (2004); Grubb et al. (2014) |
||
| OE | B. oleracea | N | N | + | leaf | Zheng et al. (2021) | |||||
| OE | B. napus | + | + | leaf/seed | resistance to Sclerotinia sclerotiorum and Botrytis cinerea | Zhang et al. (2015b) | |||||
| UGT74C1 | KO | A. thaliana | N | N | N | N | N | N | shoot | Grubb et al. (2014) | |
| UGT74B1UGT74C1 | KO | A. thaliana | – | – | – | – | – | – | shoot | Grubb et al. (2014) | |
| SOT16 | KO | A. thaliana | N | N | N | N | Nb | Nb | Klein and Papenbrock (2009) | ||
| OE | A. thaliana | +b | Klein and Papenbrock (2009) | ||||||||
a
The abbreviations of mutagenesis strategies and phenotypic alterations (N, +, –) follow Table 2. A single N, +, or – is used both for GSL alteration of a single tissue/organ and a similar alteration among multiple tissues/organs. A virgule (“/”) is used to show distinct alterations in GSL content among different tissues/organs based on the order in the column "Tissue/organ.”
b
GSL alterations were not supported by specific data but are speculated from the relevant description or calculated by indirect data evidence described in the source literature.