Fig. 6.

a In FBA method, the metabolic reaction fluxes within the boundary can be calculated by solving the equation SV = 0 (S stands for matrix and V stands for reaction rate) using linear programming, optimising or minimising an objective function, such as maximising cell growth, under given constraints. b FBA model for central carbon metabolism. The arrows indicate the flux of metabolites in a simplified representation of cell metabolism and growth. The uptake and production rates of the ith metabolite and nth metabolic phenotype are denoted as q_i/n [g/g − DW^−hr]. The FBA model is optimised by imposing the maximum growth rate, μ_m,n [hr⁻¹], as an objective function for each cell type and metabolic phenotype. b Has been adapted from Shan et al. (2018), under the terms of the Creative Commons Attribution License