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. 2023 Jul 23;19(8):67. doi: 10.1007/s11306-023-02031-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Live-cell SRS imaging of mHtt-97Q-GFP aggregates with Gln-d5 labelling. a SRS imaging of mHtt aggregates (arrowheaded, 2167 cm⁻¹, C–D on), validated by fluorescence imaging through GFP (Fluorescence). Off-resonance image at 2035 cm⁻¹ shows no signal. b Live-cell SRS images for an mHtt-97Q-GFP aggregate (arrowheaded) at Gln-d5 (2167 cm⁻¹), CH₃ (2940 cm⁻¹), and amide I (1664 cm⁻¹) channels on the same set of HeLa cells. c SRS imaging of an mHtt-97Q-GFP aggregate at 2143 cm⁻¹ by leucine-d10 (Leu-d10) labelling. d Average Sagg/Bcell from SRS images of C–D with Gln-d5 labelling (5.75 ± 1.03, n = 13); amide I (2.15 ± 0.34, n = 4); CH₃ (1.66 ± 0.14, n = 10); and C–D with Leu-d10 labelling (2.45 ± 0.33, n = 10). Error bar SD.

Reproduced from Miao and Wei (2020) under the terms of the Creative Commons Attribution License