Fig. 9.
To verify the role of TLE1 in lung adenocarcinoma. A Box plots showed the expression of TLE1 in normal and tumor tissues. B Survival curves showed survival of patients with high and low expression of TLE1. C Scatter plots showed the expression of TLE1 associated with cell cycle. D Western blot showing expression of TLE1 in HBE135-E6E7 and A549 cells. E Western blot showing TLE1 knockdown efficiency in A549 cells by independent siRNA. F Colony formation was measured in cells transfected with control or si-TLE1. Transwell assay showed cell transfer in control cells compared to TLE1 knockdown cells. G TLE1 overexpression efficiency in NCI-H2073 cell line was verified by Western blot. H Colony formation was measured in cells transfected with control or overexpressing TLE1. Transwell assay showing cell transfer in control cells compared to TLE1 overexpressing cells