Table 6.

Determination of cytotoxic effect using MTT assay towards HEK293 and HEPG2 cell lines.

Compound	HEK293	HEPG2	HT29
9	3.64 ± 0.23	1.94 ± 0.67	–
13	5.73 ± 1.64	1.92 ± 0.85	1.85 ± 0.21*
14	5.99 ± 1.05	4.2 ± 0.71	1.83 ± 0.46*
15	8.33 ± 0.75	2.53 ± 0.82	10.7 ± 3.25*
42	5.38 ± 0.35	4.88 ± 0.31	–
44	3.57 ± 0.25	4.94 ± 0.56	–
45	48.47 ± 1.50	26.64 ± 2.36	–
Etoposide	1.91 ± 0.97	5.01 ± 0.22	–
Doxorubicin	6.52 ± 0.13**	–	–

In vitro growth inhibitory activity of compounds presented as an EC₅₀ ± SD (µM) value—a concentration that inhibits 50% of mammalian cell growth.

*Antiproliferative activity of compounds 13–15 published previously²⁶.

**Antiproliferative activity of doxorubicin published previously³². In this assay, the EC₅₀ value of etoposide was recorded as a positive control.