Figure 4.

Novel allelic variations identified in the KN9204 mutant library.

(A–C) SNPs per line (A), mutated genes per line (B), and severely mutated genes per line (C) in typical lines with visible phenotypic variations and residual lines. Wilcoxon’s test was used to determine significant differences.

(D) Non-synonymous novel mutations detected in Rht-D1 (TraesKN4D01HG04200). The positions of previously reported (in blue) and novel non-synonymous mutations (in black) and stop-gained mutations (in red) are shown on a schematic diagram of Rht-D1.

(E and F) The plant height of typical mutant lines for Rht-D1. A comparison of four mutant lines with wild-type KN9204 (E) and photographs of two mutant lines (F) are shown. Note: G386∗ is heterozygous, and the mutant line containing it showed plant height trait segregation. The bar plot shows the plant heights of four mutant lines and wild-type KN9204. Dunnett’s tests were used to determine the differences between each line and the wild type. ∗P < 0.05; ∗∗P < 0.01.

(G) Large numbers of mutations were detected on the rye-derived 1BS region of KN9204. The density of SNPs (in red), annotated genes (gray), mutated genes (green), and severely mutated genes (blue) is displayed using a 2-Mb sliding window. The blue dashed box indicates the rye-derived 1BS region (Chr1B:0–283 Mb).

(H) The number of SNPs, percentage of mutated genes, and percentage of severely mutated genes in the Chr1B:0–283 Mb region in the EMS population. Numbers in the bars indicate the SNP numbers, mutated gene numbers, and severely mutated gene numbers in the Chr1B:0–283 Mb region.