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. 2023 Jul 24;11:40. doi: 10.1038/s41413-023-00262-z

Table 3.

Alkaloid-SMAs and their molecular mechanisms of action

Name Source Cell types Function Mechanisms In vivo Ref.
Neferine Nelumbo nucifera (lotus) BMMs(C57BL/6 mice) ; MC3T3 E1

OC↓

OB↑

Inhibits the NF-κB pathway OVX 153
Nuciferine Nelumbo nucifera (Lotus) BMMs(Balb/c mice) OC↓ Inhibits the NF-κB and MAPK pathways OVX; BCIO 151,152
Tetramethylpyrazine Ligusticum chuanxiong Hort. BMSCs; hPDLSCs; human chondrocytes; and human osteosarcoma cells

OC↓

OB↑

Inhibits the NF-κB and mTOR pathways;

inhibits RANKL and IL-6 signaling; activates the AMPK pathway

GIOP 25,131,186,187,264
Tomatidine Solanaceae BMMs (C57BL/6 mice)

OC↓

inflammation ↓

Inhibits the NF-κB

pathway

OVX 154
Neotuberostemonine Stemona tuberosa BMMs (C57BL/6 mice) OC↓

Inhibits the NF-κB

pathway

155
Tetrandrine Stephania tetrandra S. Moore BMMs (C57BL/6 mice)

OC↓

inflammation ↓

Inhibits the NF-κB,

PI3K/AKT, and MAPK pathways

OVX; SNM; TiP-IMAPO 156158
Berberine Berberis Linn. MC3T3-E1 cells, hMSCs, RAW264.7 cells, etc.

OC↓

OB↑

inflammation ↓

oxidative stress ↓

Inhibits the NF-κB, PI3K/Akt and NFAT pathways; activates the PKA, p38 MAPK, Wnt/β-catenin and AMPK pathways SO(SAMP6); OVX; LIOP; GIOP 188,265,266
Sanguinarine Sanguinaria Canadensis BMMs, RAW264.7 cells, MC3T3-E1 cells OC↓

Inhibits the NF-κB and

ERK pathways; activates the AMPK/Smad1 pathway

OVX 106,136
Vinpocetine Catharanthus roseus (L.) G. Don, Gen. Hist BMMs (C57BL/6 mice)

OC↓

inflammation ↓

oxidative stress ↓

Inhibits the MAPK and NF-κB pathways OVX 33
Norisoboldine Lindera aggregata (Sims) Kosterm. BMMs (male ICR mice); RAW264.7 cells OC↓ Inhibits the MAPKs/NF-κB/c-Fos/NFATc1 signaling and HIF pathways OVX 139,159
Aconine Aconitum carmichaelii Debx. RAW264.7 cells OC↓ Inhibits the NF-κB pathway - 35
Evodiamine Tetradium ruticarpum (A.Jussieu) T. G. Hartley BMMs (C57BL/6 mice) OC↓ Inhibits the NF-κB, MAPK, and calcium pathways OVX; GIOP (zebrafish) 34,71
Rutaecarpine Tetradium ruticarpum (A.Jussieu) T.G.Hartley BMMs OC↓ Inhibits the NF-κB and NFATc1 pathways - 267
Stachydrine

Leonurus heterophyllus

Sweet

BMMs (C57BL/6 mice) OC↓ Inhibits the NF-κB and Akt pathways LIOP 164
Dauricine Menispermum dauricum DC. BMMs (C57BL/6 mice)

OC↓

oxidative stress ↓

Inhibits ROS/PP2A/NF-κB signaling LIOP 189
Cytisine Leguminosae (Fabaceae) BMMs (C57BL/6 mice); RAW264.7 cells; BMSCs(C57BL/6) OC↓ Inhibits the JNK/ERK/p38-MAPK, IκB alpha/p65-NF-κB and PI3K/AKT pathways OVX 140
l-Tetrahydropalmatine Corydalis DC BMMs (C57BL/6 mice); RAW264.7 cells OC↓ Inhibits the NF-κB and MAPKs pathways OVX 141
Arecoline Areca catechu Linn.

BMMs (C57BL/6 mice);

MC3T3-E1 cells

OC↓

OB↑

Inhibits RANKL, NFATc1, and c-Fos signaling LIOP 132
Piperine Piper nigrum Linn. RAW264.7 cells; MC3T3-E1 cells

OC↓

OB↑

Inhibits p38/c-Fos/NFATc1 signaling;

activates the Wnt and AMPK pathways

OVX 81,105,137
Lycorine Lycoris radiata (L’Her.) Herb. BMMs (C57BL/6 mice) OC↓

Inhibits mROS/TRPML1/TFEB signaling;

inhibits the MAPK pathway

OVX; TiP-IMAPO; LIOP 268,269
Sinomenine Sinomenium acutum Rhed. et Wils. RAW264.7 OC↓

Activates caspase-3 activity;

downregulates IL-8/CXCR1, TLR4/TRAF6 and c-Fos/NFATc1 signaling

LIOP; BCIO 270272
Diaporisoindole E Diaporthe sp. SYSU-HQ3 BMMs (C57BL/6 mice) OC↓ Inhibits the PI3K/AKT and MAPK pathways - 167
Cinchonine Cinchona ledgeriana (Howard) Moens ex Trim. BMMs (C57BL/6 mice); RAW264.7 cells; BMSCs

OC↓

OB↑

Inhibits the NF‐κB pathway OVX; LIOP 133
Aminocoumarins Streptomyces BMMs (male ICR mice) OC↓

Inhibits the MAPK pathway;

inhibits IL-1β, TNF-α, and iNOS signaling

LIOP 273
Nitensidine A Pterogyne nitens BMMs (male ddY mice) OC↓ - 190
Theobromine Theobroma cacao L. hMSCs OB↑ -

Bone development (SD rats) during pregnancy,

lactation, and the early growth period

274
Leonurine Leonurus artemisia RAW 264.7 cells; BMMs; MC3T3-E1 cells

OC↓

OB↑

Inhibits the NF-κB and PI3K/Akt pathways;

activates the PI3K/Akt/mTOR pathway

OVX 80,275
Harmine Peganum harmala L. BMMs (male ddY mice); RAW264.7 cells;UAMS-32 cells; MC3T3-E1 cells

OC↓

OB↑

Inhibits c-Fos and NFAT c1 signaling;

activates the BMP and Runx2 pathways

OVX 120,276
Vindoline Catharanthus roseus (L.) G. Don BMMs (C57BL/6 mice)

OC↓

oxidative stress ↓

Inhibits the MAPK pathway OVX 209
Matrine and its derivatives Sophora flavescens Alt. BMSCs (rats)

OC↓

OB↑

Inhibits the NF-κB, AKT, and MAPKs pathways OVX; RMEM 277279
Securinine Flueggea suffruticosa (Pall.) Baill. BMMs (male ICR mice) OC↓ Inhibits the p38, Akt, JNK, IκB, c-Fos, and NFATc1 pathways LIOP 280
Magnoflorine Magnolia or Aristolochia BMMs (C57BL/6 mice) OC↓ Inhibits the MAPK and NF-κB pathways Ti particle-induced mice air pouch osteolysis 281
Nitidine chloride Zanthoxylum nitidum (Rutacease) and Fagara zanthoxyloides BMMs (C57BL/6 mice) OC↓ Inhibits the RANKL-induced NF-κB and NFATc1 pathways OVX 282
Largazole a cyanobacterium in the genus Symploca C2C12 cells OB↑ Activates the Runx2 and BMPs pathways

Mouse calvarial bone

formation assay; rabbit calvarial bone fracture healing model

283,284