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. 2022 Aug 5;10(5):2167–2178. doi: 10.1016/j.gendis.2022.07.006

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© 2022 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — rJAG1 treatment promoted the exosome secretion of 231 cells. (A) Schematic diagram showed the extraction procedure of exosomes from 231-Blank, 231 treated with DAPT (10 μM), 231 treated with rJAG1 (100 ng/mL), and 231B. (B) Representative images of transmission electron microscope (TEM) analysis. (C, D) Particle size analysis to determine the size distribution (C) and particle concentration (D). (E) Western blotting to determine ALIX and CD63 protein levels of exosomes from indicated cells (^∗P < 0.05, ^∗∗P < 0.01).