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Internalization of BC-3 in 5637 cells. 5637 cells were incubated with FITC-labeled BC-3 at 4 °C or 37 °C, and images were captured by (A) confocal microscopy and (B) flow cytometry (C) After treatment with proteinase K, trypsin, or EDTA, the binding of BC-3 to 5637 was analyzed by flow cytometry (scale bar = 40 μm).
