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. 2022 Aug 3;10(5):2137–2150. doi: 10.1016/j.gendis.2022.07.002

Figure 4.

Fig. 4

Endocytosis pathway analysis of BC-3 in 5637 cells. After 5637 cells were separately treated with DMSO, M-β-CD, cytochalasin D, amiloride, or chlorpromazine, fluorescent signals from the uptake level of BC-3 to 5637 were analyzed by (A) flow cytometry and (B) confocal microscopy. (C) After incubating in 5637 cells for 15 min at 37 °C, co-localization (yellow) of LysoTracker (red) and FITC (green, FITC-labeled BC-3 or FITC-labeled Library) was observed, Library as a control. (D) Enlarged view of intracellular fluorescence signals in colocalization. (E) Fluorescence intensity profile of yellow arrow regions in 5637 cells (Scale bar = 40 μm).