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. 2022 Aug 3;10(5):2137–2150. doi: 10.1016/j.gendis.2022.07.002

Figure 6.

Fig. 6

BC-3 target proteins analyzed by mass spectrometry. (A) The binding of biotin- and FITC-labeled BC-3 to 5637 cells was measured by flow cytometry, with biotin- and FITC-labeled Library as a control. (B) SDS-PAGE with silver staining was used to identify the aptamer target. Lane library, protein captured with biotin-labeled library treated sample; Lane BC-3, protein captured with biotin-labeled BC-3 treated sample. (C) Western blot analysis of the concentrations of RPS7 and β-actin in the preparations captured via Library and BC-3. (D) After incubating the ER fluorescence probe ER-tracker (red) for 0.5 h and FITC-labeled BC-3 or Library (green) for 1 h, 5637 cells displayed the colocalization (yellow) of ER-tracker and FITC-labeled BC-3. (E) Enlarged view of intracellular fluorescence signals in colocalization. (F) Fluorescence intensity profile of yellow arrow regions in 5637 cells (scale bar = 40 μm).