Figure S1.

Construction of EAU mice and unbiased classification of retinal immune cells via scRNA-seq. (A, B) Retinal anterior chamber inflammation, retinal HE staining, and corresponding clinical scores and pathology scores. Red arrows indicate conjunctival and ciliary hyperemia. Black arrows indicate inflammatory cells of eyeball sections. White arrow indicates vasculitis. n = 5. Mann–Whitney U test. ∗∗P < 0.01. (C, D) RT-qPCR and Western blot for showing the expression levels of iNOS, TNFα, and IL6 in the control and EAU groups. n = 3. Unpaired Student's t-test. ∗P < 0.05; ∗∗P < 0.01. (E) t-SNE embedding of scRNA-seq for showing different cell-type clusters in EAU retinas.