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. 2022 Oct 4;10(5):2179–2193. doi: 10.1016/j.gendis.2022.09.008

Figure 1.

Figure 1

Microglial FTO was decreased during EAU progression. (A) Retinal flat-mount staining with IBA1 (red)-labeled microglia of the control and EAU groups (n = 5). The arrows indicate activated microglia. Scale bar, 500 μm (left) and 25 μm (right). (B) CD4 (green) and IBA1 (red) staining of paraffin-embedded retinal cross-sections between the EAU mice and the control mice. The arrows indicate proliferating microglia and CD4+ T cells. Scale bar, 25 μm. (C) Retinal flow charts and quantification of CD4+ T cells (upper) and microglia (lower) at 9, 12, and 14 days of EAU. Microglia markers: CD11b and iNOS (n = 3). (D) scRNA-seq was used to detect FTO expression at 0, 14, 21, and 28 days of EAU. (E, F) The RNA and protein expression levels of FTO in the control and EAU groups (n = 3). Values are analyzed using the unpaired Student's t-test. ∗P < 0.05, ∗∗P < 0.01.