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. 2022 Oct 4;10(5):2179–2193. doi: 10.1016/j.gendis.2022.09.008

Figure 2.

Figure 2

Downregulated FTO was involved in microglia-elicited inflammation and migration in vitro. (A, B) RT-qPCR and Western blot for detecting the expression of iNOS, TNFα, and IL6 with or without LPS + IFN-γ treatment for 24 h (n = 3). (C) Griess assay for detecting NO production in the supernatant with or without LPS + IFN-γ treatment for 24 h (n = 3). (D) Transwell images and corresponding quantifications in the control group and the LPS + IFN-γ group (n = 3). Scale bar, 100 μm. (E) Representative photomicrographs of the wound-healing assay performed on HMC3 cells before and after scratching. (F) The overall m6A% level of the control group and the LPS + IFN-γ group (n = 3). (G) RT-qPCR for testing the relative mRNA expression of m6A methylase, demethylase, and readers in the control group and the LPS + IFN-γ group (n = 3). (H) FTO protein expression in the control group and the LPS + IFN-γ group (n = 3). Values are analyzed using the unpaired Student's t-test. ∗P < 0.05, ∗∗P < 0.01.